The Effectiveness of Using Saturated NaCl Solution, Sucrose, MgSO4, and ZnSO4 With Flotation Method for Examination of Soil Transmitted Helminth Infections in Elementary School Students

Sresta Azahra, Suhartini Suhartini, Fitri Nur Rica


Worm infections are a neglected tropical disease. This infection infects humans and is caused by Soil Transmitted Helminth (STH) worms, consisting of Ascaris lumbricoides, Trichuris trichiura, and hookworms. Infection control with mass drug administration and infection monitoring with sensitive and specific diagnostic methods. One of the diagnostic methods is the flotation method using a solution of NaCl, MgSO4, sucrose, and ZnSO4. The study aimed to determine the effectiveness of the use of NaCl, MgSO4, sucrose, and ZnSO4 flotation methods for examination of STH infection in elementary school students. This study was analytic observational with cross sectional design. Samples were used elementary school students' faeces to performed macroscopic and microscopic examinations of NaCl, MgSO4, sucrose, and ZnSO4 solution flotation methods. The research data was analyzed using the Kruskal Wallis with SPSS 23.0. The results showed that the number of worm eggs using ZnSO4 solution was 935 worm eggs, the average was 62,33; saturated NaCl solution as many as 564 worm eggs, an average 37,60; MgSO4 as many as 267 worm eggs, an average of 17,80; and sucrose as many as 79 worm eggs, an average of 5,27. Statistical test results obtained p value> 0.05 (p = 0,610). The conclusion is ZnSO4 solution more effective for detecting STH egg flotation method although, statistical test there is no difference between various flotation solutions for STH eggs.

Keywords: Worm infections; Soil Transmitted Helminth; worm eggs; flotation


Ngwese, M., M; Manouana, GP; Moure, PAN; Ramharter, M; Esen, M; et all. Diagnostic Techniques of Soil Transmitted Helminths: Impact on Control Measures. Trop. Med. Infect. Dis. 2020;5(93):1-17. doi:10.3390/tropicalmed5020093.

Cranston, I; Teoh, PJ; Baker, SM; Sengupta, ME; Ensink, JHJ. Evaluating The Efficacy of a Centrifugation-Flotation Method For Extracting Ascaris Ova From Soil. Trans R Soc Trop Med Hyg. 2016; 110: 400-7. doi:10.1093/trstmh/trw044.

Rosyidah, H., N; Prasetyo, H. Prevalensi infeksi cacing usus pada anak di Kampung Pasar Keputran Utara, Surabaya Tahun 2017. Journal of Vocational Health Studies. 2018; 01:117-20.

Balitbangkes (2013). Riset Kesehatan Dasar 2013. Diakses dari 013.pdf.

Rizkyta, E.,E. Gambaran Tingkat Pengetahuan Siswa Siswa Tentang Penularan Cacing pada Tubuh Manusia di SD 030 Kecamatan Muara Kaman. Universitas Muhammadiyah Kalimantan Timur; 2017.

Wardanah, H. Hubungan Tingkat Pengetahuan Anak SD Tentang Penyakit Kecacingan dengan Kejadian Kecacingan di SDN 020 Samarinda Utara. Universitas Muhammadiyah Kalimantan Timur; 2018

Setiawan, B; Nuryati, A; Sofiarahma, A. Sensitivity and Specificity of NaCl and ZnSO4 Solution Flotation Method for Examination of Worm Eggs Soil Transmitted Helminths (STH). Pakistan Journal of Medical and Health Sciences. 2020; 14(2):1-5.

Steinbaum, L; Kwong, LH; Ercumen, A; Negash, MS; Lovely, AJ; et all. Detecting and enumerating soil transmitted helminths eggs in soil: New method development and results from field testing in Kenya and Bangladesh. Plos Negl Trop Dis. 2017:11(4): e0005522.

Amoah, ID; Sigh, G; Stenstrom, TA; Reddy, P. Detection and Quantification of Soil Transmitted Helminths in Environmental Samples: A review of current state of the art and future perspectives. Acta Tropica. 2017: 169: 187-201.

Aswita, Rini. Pengaruh faktor perilaku dan lingkungan terhadap penyakit infeksi kecacingan pada ibu hamil di Kabupaten Langkat. Universitas Sumatera Utara; 2020.

Pouillevet, H.; Dibakou, S.E; Ngoubangoye, B; Poirotte, C.; Charpentier,M.J.E. A comparative study of four methods for the detection of nematode eggs and large protozoan cysts in Mandrill Faecal Material. Folia Primatol. 2017;88:344-57. doi: 0.1159/000480233.

Setiawan, B.; Syayyidah, G.A.D; Hardisari, R.; Widada, S.T; Nuryati, A. Jumlah telur Soil Transmitted Helminth (STH) pada metode sedimentasi dan flotasi. Jurnal Kesehatan Lingkungan. 2022; 12(1):142-45. doi: 10.47718/jkl.v10i2.1184.

Savitrie, DWR; Sudaryanto; Ningrum, FH; Kisdjamiatun, RA. Comparative Effectiveness and Optional Period of The Flotation Method Using NaCl, MgSO4, and ZnSO4 For The Diagnostic of Soil Transmitted Helminths. Jurnal Media Medika Muda. 2014.

Wong, M.S.; Bundy, D.A.P. Quantitative assessment of contamination of soil by the eggs of Ascaris lumbricoides and Trichuris trichiura. Transactions of the royal society of tropical medicine and hygiene. 1990;84:567-70.

Ines, A.J.; Pacheco, F.T.F.; Pinto, M.C.; Mendes, P.S.A.; Ribeiro, H.C.; Soares, N.M.; Teixeira, M.C.A.; Condordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites. Biomedica. 2016;36: 519-24. doi:

Bowman, D.D.; Little, M.D., Reimers, R.S. Precision and accuracy of an assay for detecting Ascaris eggs in various biosolid matrices. Water Research. 2003;37: 2063-72. doi:10.1016/S0043-1354(02)00597-3.

Cranston, I.; Teoh, P.J.; Baker, S.M.; Sengupta, M.E.; Ensink, J.H.J. Evaluating the efficacy of centrifugation-flotation method for extracting Ascaris ova from soil. Trans R Soc Trop Med Hys. 2016; 110: 400-407. doi:10.1093/trstmh/trw044.

Auliana; Puspawati; Arsyad, M. Comparison of NaCl Usage Saturated with ZnSO4 Saturated Using Flotation Methods on Number of Shaving Eggs Soil Transmitted Helminth (STH) KTI. Akademi Kesehatan Borneo Lestari Banjarbaru; 2017.

David, E.D., Lindquist W.D. Determination of the specific gravity of certain helminth eggs using sucrose density gradient centrifugation. J. Parasitol. 1982: 68 (5); 916-19.

Amoah, I.D., Reddy, P., Stenstrom, T.A. Effect of reagents used during detection and quantification of Ascaris suum in environmental samples on egg viability. Water Science & Technology. 2017;76(9):2389-400. doi: 10.2166/wst.2017.324.

Rosnia, D., W. Sudaryanto, Ningrum, F., H. Kisdjamiatun. Comparative effectiveness and optional period of the flotation method using NaCl, MgSO4, and ZnSO4 for the diagnostic of Soil Transmitted Helminths. Jurnal Media Medika Muda. 2014.



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