Potensi Ekstrak Rumput Kebar (Biophytum petersianum Klotzsch) Dalam Mempertahankan Jumlah Sel Sertoli Mencit (Mus musculus) Model Diabetes Melitus

Etik Yuliarini Widodo, Reny I’tishom, Bambang Purwanto

Abstract


Background: Hyperglycemia is a common effect of uncontrolled DM. The main impact of DM on male infertility is the result of hyperglycemia which causes an increasing in free radicals which cause oxidative stress in the testes, and then attacks Sertoli cells. Increasing of free radicals will cause disruption and damage to this cell, including apoptosis and atrophy and then caused decreasing in the number of Sertoli cells. Kebar grass contains antioxidants compounds, such as flavonoids and vitamin E. There are synergy effects between vitamin E and flavonoids which reinforce both in working to improve infertility as an antioxidant. Objective: To compared the number of Sertoli cells between groups which receiving extract kebar grass (Biophytum petersianum Klotzsch) and the control group in diabetes mellitus mice (Mus musculus) model. Methods: The research subjects used DM male mice which were divided into 5 groups: 2 control groups (K- and K+) and 3 treatment groups (P1, P2, P3). Day 1 to day 5, all groups were induced by STZ. The 11th day to the 45th day, the control group (K+) is given metformin 2 mg/head /day, the P1, P2 and P3 groups is given kebar grass with dose of 67,5; 135 and 270 mg/kg/day and metformin 2 mg/head/day. The 11th day to the 31th day, the control group (K-) is given CMC-Na 1% suspension. The 46th day is done surgery and sampling testicular tissue. Examination of the number of Sertoli cells is done through examination of the histological picture of testicular tissue that has been given Haematoxylin-Eosin (HE) staining. Results: Statistical tests showed that there was significant differences with a value of p = 0.000 (<0.05) in the number of Sertoli cells. Mean and standard deviation of Sertoli cells in each group were K- = 8.63 ± 0.50; K+ = 9.87 ± 1.52; P1 = 11.40 ± 0.77; P2 = 14.75 ± 1.97 and P3 = 14.97 ± 2.00. Conclusion: Kebar grass extract can maintain the number of Sertoli cells in diabetes mellitus mice models.
Keywords: Kebar grass extract; Sertoli cells; diabetes mellitus

ABSTRAK

Latar belakang: Hiperglikemia adalah efek umum dari DM yang tidak terkontrol. Dampak utama DM terhadap infertilitas pria adalah kondisi hiperglikemik menyebabkan peningkatan radikal bebas dan terjadi stres oksidatif pada testis, kemudian menyerang sel Sertoli. Peningkatan radikal bebas memicu terjadinya gangguan dan kerusakan pada sel-sel tersebut, seperti apoptosis dan atrofi serta terjadi penurunan jumlah sel. Rumput kebar mengandung senyawa yang berperan sebagai antioksidan, seperti flavonoid dan vitamin E. Terdapat efek sinergi antara vitamin E dan flavonoid yang memperkuat kerja keduanya dalam memperbaiki gangguan infertilitas sebagai antioksidan. Tujuan: Membandingkan jumlah sel Sertoli mencit (Mus musculus) model diabetes melitus antara kelompok yang mendapat ekstrak etanol rumput kebar (Biophytum petersianum Klotzsch) dan kelompok kontrol. Bahan dan cara: Subyek penelitian menggunakan mencit jantan DM, dibagi 5 kelompok yaitu 2 kelompok tatist (K- dan K+) dan 3 kelompok perlakuan (P1, P2, P3). Hari ke-1 sampai hari ke-5, semua kelompok diinduksi STZ. Hari ke-11 sampai hari ke-45, kelompok tatist (K-) disonde tatisti CMC-Na 1%, kelompok tatist (K+) disonde metformin 2 mg/ekor/hari, kelompok P1, P2 dan P3 disonde rumput kebar dosis 67,5, 135, 270 mg/kg BB/hari dan metformin 2 mg/ekor/hari, Hari ke – 46 dilakukan pembedahan dan pengambilan sampel jaringan testis kanan dan kiri. Pemeriksaan jumlah sel Sertoli dilakukan melalui pengamatan gambaran histologi jaringan testis yang telah diberikan pewarnaan Haematoxylin-Eosin (HE). Hasil: Uji statistik menunjukkan bahwa terdapat perbedaan bermakna dengan nilai p = 0.000 (< 0,05) pada jumlah sel Sertoli. Mean dan standar deviasi sel Sertoli pada tiap kelompok adalah K- = 8,63 ± 0,50; K+ = 9,87 ± 1,52; P1 = 11,40 ± 0,77; P2 = 14,75 ± 1,97 and P3 = 14,97 ± 2,00. Kesimpulan: Ekstrak rumput kebar dapat mempertahankan jumlah sel Sertoli pada mencit model diabetes melitus.
Kata kunci : ekstrak rumput kebar; sel Sertoli; diabetes melitus

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DOI: http://dx.doi.org/10.33846/sf11311

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